Diaporthe eres|109792 |BNCC

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Diaporthe eres

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the plate and onto the surface of the agar; ⑤ Cultivate the flat plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	25-28 ℃, 5-7 days, Aerobic,
Storage conditions	-80 ℃
Safety level	1
morphology	Spread growth, filamentous, irregular edges, opaque, white on the front, gray white on the back, raised in the middle, rough surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

Phomopsis sp.

Storage conditions: 2~8 ℃

No. 109792

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Growth conditions: 28°C, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability,in 5 days plate colony is obvious
colony morphology:	Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white, The hyphae are dense and vigorous, spreading to the edge of the plate, and the back of the medium is yellowish.
Conclusion:	good viability, no abnormal colony morphology, qualified

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