BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ℃, 5-7 days, aerobic |
| Subculture procedure | ① Prepare one 90mm solid Agar plate, one inoculation shovel and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Small filamentous fungi have distinct colonies on a comprehensive PDA culture medium, with flat and fluffy colonies. They appear white in the early stages and pink, red, yellow brown, or gray brown in the later stages. Sometimes, multiple colors can be produced simultaneously on one colony, spreading and growing towards the edge of the Agar plate. The back of the culture medium is light yellow |
| Sharing mode | Public welfare sharing |
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