Staphylococcus aureus|119060 |BNCC

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Epicoccum nigrum Link

Subculture procedure	① Prepare one 90mm solid Agar plate, one inoculation shovel and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have distinct colonies on a comprehensive PDA culture medium, with flat and fluffy colonies. They appear white in the early stages and pink, red, yellow brown, or gray brown in the later stages. Sometimes, multiple colors can be produced simultaneously on one colony, spreading and growing towards the edge of the Agar plate. The back of the culture medium is light yellow
Sharing mode	Public welfare sharing

Epicoccum nigrum Link

Storage conditions: 2~8 ℃

No. 119060

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, integrated PDA, 7-10 days. Comprehensive PDA: 1.0L potato cooking liquid, 20.0g glucose, 3.0g KH2PO4, 1.5g MgSO4 7H2O, trace amount of vitamin B1, 20.0g agar, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:
(1) Prepare 1-2 pieces of PDA plates;
(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;
(4) Lay flat the small pieces to the center of the agar plate;
(5) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 7 days, plate colonies are obvious
colony morphology:	small filamentous fungi have obvious colonies on comprehensive PDA culture medium, with flat colonies, cotton wool-like colonies, white at the initial stage, pink, red, yellowish brown or grayish brown at the later stage, sometimes several colors can be produced on one colony at the same time, spreading and growing to the edge of the plate, and the back of the culture medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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