Blood red thrombus fungus|121779 |BNCC

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Trametes sanguinea (L.) Lloyd

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The mycelium is dense and flat, the colony is blood red, and the reverse side is yellow
Sharing mode	Public welfare sharing

Trametes sanguinea (L.) Lloyd

Storage conditions : 2~8 ℃

No. 121779

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, 5-7 days, comprehensive PDA agar: potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0±0.2. 121°C , 15min sterilization. Potato boiling liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:
(1) Prepare 1-2 pieces of PDA plates;
(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;
(4) Lay flat the small pieces to the center of the agar plate;
(5) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 5-7 days strain layer become obvious
colony morphology: (above)	filamentous fungi have obvious colonies on comprehensive PDA medium, with white hyphae, dense and low-level hyphae, vigorous spread and growth of hyphae, producing a large number of white spores.
Conclusion:	good viability, no abnormal colony morphology, qualified

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