Beauveria bassiana|122066 |BNCC

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Beauveria bassiana (Bals.-Criv.) Vuill.

Subculture procedure	① Prepare 1-2 of the above tablets; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	25~28 ℃, 3-5 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Spreading growth, filamentous, white to pale on the obverse.
Sharing mode	Public welfare sharing

Beauveria bassiana

Storage conditions: 2~8 ℃

No.: 122066

Product format: 14mm test tube bevel

Validity period: 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, integrated PDA agar, 5-7 days. Comprehensive PDA agar: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g, MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0± 0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability，in 5 days Plate colony is obvious
colony morphology:	Small filamentous fungi, with obvious colonies on integrated PDA medium, white colonies, Beauveria bassiana produces conidia, The surface of the conidia of Beauveria bassiana has rough wrinkles, dense and vigorous, spreading to concentric circles on the edge of the plate
Conclusion:	good viability, no abnormal colony morphology, qualified

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