Specialized type of Fusarium oxysporum|111420 |BNCC

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Fusarium oxysporum f.sp. lagenariae Matuo & I. Yamam.

Subculture procedure	① Dissolve the cryovials in a water bath (mold at 30 ℃, bacteria at 37 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; Aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi with distinct colonies on PDA medium, with pink centers and white edges, dense and vigorous growth, spreading towards concentric circles on the edge of the plate, and not producing spores
Sharing mode	Public welfare sharing

Fusarium oxysporum f.sp. lagenariae

Storage conditions: 2~8 ℃

No. 111420

Product format : agar slant in 14mm test tube

Validity period : growing culture in 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, PDA,5-7 days, PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (liquid medium does not contain), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page),the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 5-7 days plate colony is obvious
colony morphology: (above)	small filamentous fungus with obvious colonies on PDA medium, pink colony center and white edge, dense and vigorous, spreading to concentric circles at the edge of the plate, without spores
Conclusion:	good viability, no abnormal colony morphology, qualified

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