Bacillus subtilis subsp. subtilis

Storage conditions: 2~8 ℃

No. 132897

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level:  1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions: 37℃, aerobic, nutrient gravy agar, 18-24h. Nutrient gravy agar: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 15.0g, distilled water 1.0L, pH 7.0. [Note] Adding 5mg MnSO4·H2O when culturing Bacillus is beneficial to produce spores. pH7.0. 121°C, 15min.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; 
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows: