Mao Tou Gui Umbrella|109829 |BNCC

Essential Information Certificate Related Products

Coprinus comatus

Subculture procedure	Agar slant; Agar plate, None; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the plate and onto the surface of the agar; ⑤ Cultivate the flat plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	Liquid nitrogen
Safety level	1
morphology	Agar plate, with white hyphae that are sparse and vigorous, spreading and growing towards the edge of the agar plate, and do not produce spores
Sharing mode	Public welfare sharing

Coprinopsis atramentaria

Storage conditions: 2~8 ℃

No.: 109829

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, integrated PDA. Comprehensive PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, glucose 20.0g, vitamin B1 10mg, agar 20g,pH natural. 121 ℃,15min. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page),the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions for 7-10 days. You can also contact customer service for information "Inclined Transfer Method-Filamentous Fungi".

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 7 days plate colony is obvious
colony morphology:	small filamentous fungi are obviously visible on the integrated PDA agar medium. The flesh is white at the beginning and gray at the end. The gills are dense, crowded with each other, free, unequal in length, starting gray-white to gray-pink, without spores.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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