BeNa Culture Collection
info@bncc.com
| Subculture procedure | ① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the plate and onto the surface of the agar; ⑤ Cultivate the flat plate under the above cultivation conditions, and the strain can be used once it grows. |
| Growth conditions | 25-28 ℃; 5-7 days; Aerobic |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | A small filamentous fungus that forms distinct colonies on comprehensive PDA medium. The mycelium is white, cotton-like in texture, dense, and vigorous, spreading concentrically toward the edges of the plate. No spore production is observed. |
| Sharing mode | Public welfare sharing |
Trametes versicolor (L.) Lloyd
Storage conditions: 2~8 ℃
number: 112791
product form:agar slant in 14mm test tube
Validity period: growing culture in 30 days
Biosafety level: 1, handle in ultra-clear table or safety cabinet
Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :25-28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Recovery steps:
(1)Prepare 1-2 pieces of PDA plates;
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page),the size of square pieces is 0.5 × 0.5cm2;
(4)Lay flat the small pieces to the center of the agar plate;
(5)put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test result |
| viability | good viability, in 5-7 days plate colony is obvious |
| colony morphology: |
small filamentous fungi with obvious colonies on comprehensive PDA medium, white hyphae, cotton lumpy, dense and vigorous, facing the flat edge concentric circles spread and grow without spores. |
| Conclusion: | good viability, no abnormal colony morphology, qualified |
Water quality fecal coliform positive quality control samples (Paper Rapid Method)
BNCC360408
Reference material of total bacterial count in food
BNCC359084
Water quality total coliform negative quality control sample (paper rapid method)
BNCC360413
Biochemical ldentification Kit for salmonella
BNCC375994
Quantitative strains of staphylococcus aureus subsp. aureus Rosenbach
BNCC353786
Quantitative strains of aspergillus niger
BNCC362554
- English
- Japanese
