BeNa Culture Collection
info@bncc.com
| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Growth conditions | 28 ℃; 5-7 days; aerobic |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Small filamentous fungus; colonies are distinct on comprehensive PDA medium, with grayish-white, dense, and vigorous hyphae spreading toward the agar plate edges in concentric circles. |
| Sharing mode | Public welfare sharing |
Fusarium oxysporum
Storage conditions : 2~8 ℃
No. : 117677
Product format : agar slant in 14mm test tube
Validity period: 30 days
Biosafety level : 1, handle in ultra-clear table or safety cabinet
Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA agar medium: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Recovery steps:
(1)Prepare 1-2 pieces of PDA plates;
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page),the size of square pieces is 0.5 × 0.5cm2;
(4)Lay flat the small pieces to the center of the agar plate;
(5)Put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test result |
| viability | good viability, in 7 days plate colony is obvious |
| colony morphology: | Small filamentous fungi have obvious colonies on the integrated PDA medium, with gray-white hyphae, dense and vigorous, spreading to concentric circles on the edge of the plate. |
| Conclusion: | good viability, no abnormal colony morphology, qualified |
Fecal coliform standard quality control sample (Multitube fermentation method)
BNCC359289
Quality control sample of total bacterial count in drinking water
BNCC377988
Biochemical ldentification Kit for Staphylococcus aureus
BNCC370063
Quantitative strains of Staphylococcus aureus
BNCC381462
Quantitative strains of escherichia coli (Migula) Castellani and Chalmers
BNCC356000
Quantitative strains of aspergillus brasiliensis Varga et al.
BNCC353778
- English
- Japanese
