Penicillium candidum|109699 |BNCC

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Paecilomyces farinosus (Holmsk.) A.H.S. Br. & G. Sm.

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃, 10-15 days, aerobic
Storage conditions	-80 ℃ freezer freezing method; Vacuum freeze-drying method; Regular transplantation method
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, with white and dense hyphae in the early stage, and later produce yellow spores
Sharing mode	Public welfare sharing

Paecilomyces farinosus

Storage conditions: 2~8 ℃

No. : 109699

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions : 28℃, aerobic, comprehensive PDA, 10-15 days, comprehensive PDA: potato boiling liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0±0.2. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 10 days plate colonies are obvious
colony morphology: (above)	small filamentous fungi have obvious colonies on comprehensive PDA medium, with white hyphae at the initial stage, dense and vigorous, and yellow spores at the later stage.
Conclusion:	good viability, no abnormal colony morphology, qualified

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