Botrytis cinerea|121059 |BNCC

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Botryosphaeria dothidea

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃, 3-5 days, aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, gray white in color, dense and vigorous mycelium, spreading and growing towards the edge of the Agar plate, and dark yellow on the back of the medium
Sharing mode	Public welfare sharing

Botryosphaeria dothidea

Storage conditions : 2~8 ℃

No. : 121059

Product format : agar slant in 14mm test tube

Validity period : 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄ 3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page),the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions for 5-7 days. You can also contact customer service for information "Inclined Transfer Method-Filamentous Fungi".

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 5 days plate colony is obvious
colony morphology:	small filamentous fungi, colonies are obviously visible on comprehensive PDA culture medium, hyphae are brown, the center is gray-black, cotton wool-shaped, dense, and the back is dark blue.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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