Botrytis cinerea|110179 |BNCC

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Cladosporium sp.

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	25-28 ℃, 5-7 days, aerobic.
Storage conditions	2-8 ℃
Safety level	1
morphology	Filamentous fungi have obvious colonies on comprehensive PDA medium, dense and flat hyphae, and almost no spread. The initial hyphae are white, and later gray spores are produced
Sharing mode	Public welfare sharing

Cladosporium sp.

Storage conditions: 2~8 ℃

No. 110179

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions : 25-28°C, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: 1.0L potato cooking liquid, 20.0g glucose, 3.0g KH2PO4, 1.5g MgSO4 7H2O, trace amount of vitamin B1, 20.0g agar, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 5days strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are dense and low, and there is almost no spread. The initial hyphae are white, and the later gray spores are produced.
Conclusion:	good viability, no abnormal colony morphology, qualified

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