Staphylococcus aureus|340652 |BNCC

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Staphylococcus aureus

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, golden yellow on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Staphylococcus aureus

Storage conditions: 2~8 ℃

No.: 340652

product format: freeze dried, 200ul, or 1agar slant in 14mm test tube x 1

Validity period: Freeze-dried tube for 24 months, bevel for 30 days

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Activation/passage:37°C, aerobic, nutrient agar/gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, 18-24 hours

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, 20h,NB bacterial fluid is turbid, NA strain layer is obvious
colony morphology	size: moderate color: golden yellow shape: round edge: full edge wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: middle bulge
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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