Chinese Ganoderma (Ganoderma lucidum)|143276 |BNCC

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Ganoderma sinense

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 10-15 days; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Spread growth, filamentous, irregular edges, opaque, gray white front, flat shape, rough surface, wrinkled surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

Ganoderma sinense

Storage conditions: 2~8 ℃

No. 143276

Product format: agar slant in 14mm test tube

Validity period: growing culture in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:28 ℃, aerobic, integrated PDA agar, 10-15 days. Comprehensive PDA agar: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g, MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 10-15 days plate colony is obvious
colony morphology	Small filamentous fungi have obvious colonies on the integrated PDA medium, white hyphae, dense and low flat, and spread to the edge of the plate.
Conclusion	good viability, no abnormal colony morphology, qualified

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