BeNa Culture Collection
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| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Growth conditions | 25-28 ℃, 5-7 days; aerobic; |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Filamentous fungus. Colonies on Czapek agar are distinct, with dense, low-lying, non-spreading mycelium, and produce yellow spores. |
| Sharing mode | Public welfare sharing |
Eurotium cristatum
Storage conditions: 2~8 ℃
No. 146559
Product format: freeze dried, 200ul
Validity : 6 years
Biosafety level : 1 , handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:25-28 ℃, aerobic, Cha's agar, 5-7 days. Cha's agar: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water, pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Recovery steps:
(1)Prepare the above 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| Item | test results |
| viability: | good viability, in 5days strain layer become obvious;colony is typical on the streaked plate |
| colony morphology: (above) | filamentous fungus, with obvious colonies on Cha's agar, dense hyphae, low flat and no spread, producing yellow spores. |
| Conclusion: | good viability, no abnormal colony morphology, qualified |
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