Lactobacillus plantarum subspecies|185362 |BNCC

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Lactobacillus plantarum subsp. plantarum

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 48-72h; microaerophilic (3% -5% O2, 10% CO2);
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony diameter is 1-2 mm, circular with neat edges, opaque, white on the obverse, convex in the center, smooth and shiny surface, moist texture, easily picked, Gram-positive (blue-violet), rod-shaped, purity: pure.
Sharing mode	Public welfare sharing

Lactobacillus plantarum subsp.

Storage conditions: 2~8 ℃

No.: 185362

Product format : freeze dried,200ul

Validity period: Freeze-dried tube for 24 months

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :37 ℃, anaerobic, MRS medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1000mL. pH6.2±0.2. 121 ℃,15min.

Recovery steps:

(1) Prepare 2 above mentioned MRS plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 40h MRS plate colony is obvious
colony morphology:	Size: Medium Color: Milk White Shape: Round Edge: Neat Edge Wet and dry: Wet and smooth: smooth and bright Transparency: Opaque Protuberance: Bulge
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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