Streptococcus genus|189361 |BNCC

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Streptococcus sp.

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 48-72h; 5% CO2
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Streptococcus sp.

Storage conditions: 2~8 ℃

No. 189361

Product format: freeze dried, 200ul

Validity: 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Gorwth conditions: 37 ℃,5% CO2, Columbia blood plate (ready-to-use type),48-72h.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 48-72 days.

Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows:

Item	test results
viability:	good viability, in 3days strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque color: white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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