Avermectin Streptomyces|152966 |BNCC

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Streptomyces avermitilis

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃, 3-5 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony has a diameter of 2-4mm, circular, serrated edges, opaque, yellow in the early stage and white spores in the later stage. The surface is rough, wrinkled, hard in texture, dry in texture, rod-shaped, purity: pure
Sharing mode	Public welfare sharing

Streptomyces avermectin

Storage conditions : 2~8 ℃

No. : 152966

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, safety cabinet operation

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, ISP-2,3-5 days. Yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5days strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 2-4mm Shape: Round Edge: Jagged Transparency: Opaque Color: White Protrusion: Middle Raised Surface: Rough Gray and Dark Texture: Hard and Dry
Conclusion:	good viability, no abnormal colony morphology, qualified

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