Purple Monascus|195630 |BNCC

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Monascus purpureus

Subculture procedure	① Prepare 1-2 fresh tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi with obvious colonies on malt extract agar medium, purple red colonies, radial folds on the medium, no exudate or soluble pigments
Sharing mode	Public welfare sharing

Monascus purpureus

BNCC No.：195630

Storage conditions: 2~8 ℃

Product format：freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30 ℃, aerobic, 3-7 days, malt juice agar: malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 3-7 days plate colony is obvious
colony morphology: (above)	small filamentous fungi with obvious colonies on wort agar medium, colonies are purple-red, with radial folds on the culture medium, no exudate and soluble pigment.
conclusion:	good viability, no abnormal colony morphology, qualified

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