Streptococcus pyogenes|153538 |BNCC

Essential Information Certificate Related Products

Rhodococcus ruber

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	30 ℃, 2-3 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2 mm,Shape: Circular,Margin: Undulate (wavy),Transparency: Opaque,Color: Orange-red,Elevation: Flat,Surface: Rough,Texture: Dry
Sharing mode	Public welfare sharing

Rhodococcus ruber

Storage conditions : 2~8 ℃

No. : 153538

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28-30 ℃, aerobic, Streptomyces medium No. 2, 48-72h, Streptomyces medium No. 2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2 ± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 48-72 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 48-72h plate colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Wavy Transparency: Opaque Color: Orange Red Height: Flat Surface: Rough Texture: Dry
Conclusion:	good viability, no abnormal colony morphology, qualified

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