Layered Fusarium|195647 |BNCC

Essential Information References Certificate Related Products

Fusarium proliferatum

Subculture procedure	① Dissolve the Frozen vial in a water bath (fungi at 30 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃, 3-5 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi have obvious white colonies and dense hyphae on the comprehensive PDA medium, spreading and growing towards the edge of the Agar plate. The back of the medium is orange yellow
Sharing mode	Public welfare sharing

1.Description

1. Name：Fusarium proliferatum

2. BNCC No.：195647

3. Biosafety level： 4

2.Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Medium composition: potato extract 1.0L, glucose 20.0g, agar 15.0g,pH natural. [Note] Potato extract: 200g of peeled potato is taken, cut into small pieces, add 1.0L of water to boil for 30min, filter out potato pieces, and supplement the filtrate to 1.0L.

2. Atmosphere：aerobic

3. Temperature： 25 ℃

4.Notes

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

Download certificate