Bacillus licheniformis|185404 |BNCC

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Bacillus licheniformis

Subculture procedure	① Prepare 1-2 plates as described above.② Within a biosafety cabinet, open the vial and flame the top briefly with an alcohol lamp, then immediately add sterile water to break the seal. Use forceps to crush the vial.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Growth conditions	30 ℃; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2 mm,Shape: Irregular,Margin: Irregular,Transparency: Opaque,Color: Pale yellow,Elevation: Flat,Surface: Rough and dull,Texture: Dry
Sharing mode	Public welfare sharing

Bacillus licheniformis

Storage conditions : 2~8 ℃

No. : 185404

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30 ℃, aerobic, nutrient agar medium, 18-24h. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 18 hours liquid medium become turbid,strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Irregular Edge: Untidy Transparency: Opaque Color: Light Yellow Height: Flat Surface: Rough Gray Texture: Dry
Conclusion:	good viability, no abnormal colony morphology, qualified

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