Staphylococcus aureus subspecies|238481 |BNCC

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Staphylococcus aureus subsp. aureus Rosenbach

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet and follow the arrow indication on the cap of the penicillin bottle to open the strain and reconstitution solution; ③ Add 1mL of the complex solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Take 200 μ L of bacterial suspension separately and inject it into two plates, apply evenly; ⑤ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, golden yellow on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), cocci
Sharing mode	Public welfare sharing

Staphylococcus aureus

BNCC No.: 238481

Product format: Freeze dried 200ul , or agar slant in 14mm test tube

Storage conditions: 2~8 ℃

Validity period: Freeze-dried tube for 6 years, iagar slant for 30 days

Biosafety level: 2,handle in safety cabinet

Notes: If any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, nutrient agar/gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:
(1)Prepare a flask of NB liquid media or two NA agar plates.

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 37℃ for 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
Viability:	good viability,in 20h NB bacterial fluid is turbid, NA plate colony is obvious
colony morphology:	size: smaller color: golden yellow shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: middle bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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