MIT Hb1 Helicobacter bilis

Storage conditions: 2~8 ℃
No. : 246687
Product format : freeze dried, 200ul
Validity: 6 years
Biosafety level : 2, handle in bio-safety cabinet 
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :37 ℃, micro aerobic, Colombian blood plate (ready-to-use).
Recovery steps :
① Prepare 1-2 of above mentioned plates; ;
② sterilize of the plate surface and open it in the biosafety cabinet, 
③ draw 0.5ml of sterile water into the plates, and scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension;
④ draw the bacteria suspension with a pasteur pipette into a fresh plate, and spread it evenly; 
⑤ Place the plate under the above culture conditions for cultivation.
Recovery record:  According to the recovery instructions, single colony is observed by marking, and the results of the recovery are reported as follows: