Occasional subspecies of sporadically branched bacteria|186102 |BNCC

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Mycobacterium fortuitum subsp. fortuitum

Subculture procedure	① Prepare 1-2 plates as specified above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol lamp, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Growth conditions	37 ℃; 48-72h; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies measure 0.5-1 mm in diameter, exhibit an irregular shape and irregular margins, and are opaque. They appear pale yellow on the surface, display a flat morphology, and have a rough surface with a dry texture. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli).
Sharing mode	Public welfare sharing

1. Description

1. Name: Mycobacterium fortuitum subsp.

2. BNCC No.: 186102

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Nutritious gravy agar: peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4 .H2O when culturing Bacillus,which is beneficial to spore production.

2. Atmosphere: aerobic

3. Temperature: 37 ℃,24-48h

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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