Animal Bifidobacterium|186305 |BNCC

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Animal Bifidobacterium-BNCC
Animal Bifidobacterium-BNCC
  • BNCC
  • Bifidobacterium animalis-BNCC
  • Bifidobacterium animalis-BNCC

Bifidobacterium animalis

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  • number：186305
  • Form：
    The colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They appear grayish-white on the surface, with a raised center and a smooth texture. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli).
Standard strain Quantitative strain DNA extraction
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Bifidobacterium animalis
Subculture procedure ① Prepare 1-2 plates as specified above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol lamp, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Growth conditions 37 ℃; 24-48 hours; anaerobic
Storage conditions 2-8 ℃
Safety level 1
morphology The colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They appear grayish-white on the surface, with a raised center and a smooth texture. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli).
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1. Description 

1. Name: Bifidobacterium animalis

2. BNCC No.: 186305

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions: 

1. BBL liquid medium: peptone 15.0g, glucose 20.0g, yeast extract 2.0g, soluble starch 0.5g, sodium chloride 5.0g,L-cysteine 0.5g, tomato extract 5.0g, liver extract 2.0g, Tween 80 1.0g, distilled water 1L,pH6.6-7.0. 115 ℃,20min.

2. Atmosphere: anaerobic

3. Temperature:  37 ℃,48h

4. Notes: 

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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