BeNa Culture Collection
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| Culture medium | BNCC352254 |
| Description | BBL agar medium |
| Composition | Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; anaerobic |
| Subculture procedure | ① Prepare 1-2 plates as specified above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol lamp, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They appear grayish-white on the surface, with a raised center and a smooth texture. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli). |
| Sharing mode | Public welfare sharing |
KMS11(KMS11)
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Py230(Py230)
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