Staphylococcus aureus|186335 |BNCC

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Staphylococcus aureus

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet and follow the arrow indication on the cap of the penicillin bottle to open the strain and reconstitution solution; ③ Add 1mL of the complex solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Take 200 μ L of bacterial suspension separately and inject it into two plates, apply evenly; ⑤ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, golden yellow on the front, raised in the middle, smooth and bright on the surface, moist texture, and easy to handle
Sharing mode	Public welfare sharing

Staphylococcus aureus

No.: 186335

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if it is overdue, it will be regarded as good receiving goods. Dry powder shall not be retained after one use, and can be used after 1~2 generations of activation. please refer to the attached page for details of inclined plane and bacterial solution. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:37 ℃, aerobic, nutrient agar medium, 18-24h. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:

① Prepare a test tube containing 5~10mL of liquid culture medium and 2 plates;

② Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ Suck 0.5mL of liquid culture medium into lyophilized tube, fully dissolve and return to liquid test tube again, and mix well;

④ Absorb 0.2mL of bacterial suspension into the plate, coat evenly, repeat twice to obtain two plates;

⑤ Place all the liquid test tubes and plates under the above culture conditions, and the strains can be used when they grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 20h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	size: 2-4mm shape: round edge: neat transparency: opaque color: milky white uplift: middle raised surface: bright and smooth texture: moist and easy to stir
conclusion	good viability, no abnormal colony morphology, qualified

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