Swiss Lactobacillus|187926 |BNCC

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Lactobacillus helveticus

Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 24-48 hours; Micro aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, irregular in shape, irregular in edges, opaque, gray white on the front, flat in shape, gray on the surface, easy to pick up, G+(blue purple), rod-shaped
Sharing mode	Public welfare sharing

Lactobacillus helveticus

Storage conditions: 2~8 ℃

No.: 187926

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level:1

Receiving notice: if any abnormal situation such as damage is found on the day of receipt, please contact customer service within 24 hours. Dry powder cannot be retained once used up. please refer to the attached page for details of the use of bacterial liquid. Please operate in strict accordance with this instruction,otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0l. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

1. Prepare one sterile test tube liquid culture medium, 25mL of 18mm large test tube liquid, and plug it with breathable silicone plug;

2. After the outer wall of the strain tube is disinfected, in a sterile environment, use a small grinding wheel to draw a circle at 1/2 of the tube body, and then break it;

3. Absorb about 0.5mL of liquid culture medium into the strain tube, blow gently, fully dissolve and mix well;

4. Suck out all the dissolved solution of the bacterial powder and return it to the test tube, and plug the silicone plug;

5. Place the test tube in an incubator and culture under the above specified conditions. The culture solution is obviously turbid or precipitated, and the activation is completed.

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