Legionella pneumophila subspecies|312197 |BNCC

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Legionella pneumophila subsp.pneumophila Brenner et al.

Culture medium	BNCC360928
Description	Buffered Charcoal Yeast Extract Agar
Composition	Yeast Extract Powder:10.0g, Activated Charcoal:2.0g, Agar:13.0g, α-Ketoglutarate Monopotassium Salt(C₅H₅KO₅):1.0g, ACES Buffer:10.0g, Potassium Hydroxide(KOH):2.8g, Ferric Pyrophosphate(Fe₄(P₂O₇)₃):0.25g
Growth conditions	37 ℃; 24-48h；5%CO2；
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	Colony diameter 0.5-2 mm, circular with irregular margins, opaque, grayish-white on the obverse, convex in the center, smooth and glistening surface, moist and viscous texture, Gram-negative (red), rod-shaped, purity: pure.
Sharing mode	Public welfare sharing