Salmonella Enteritidis subsp. Enteritidis LT2|316132 |BNCC

Essential Information Related Products

Salmonella enterica subsp. enterica

Culture medium	BNCC353940
Description	3.5% Sodium Chloride Agar Medium
Composition	Beef Extract:3.0g, Peptone:10.0g, NaCl:35.0g, Agar:15.0g, pH:7.2-7.4(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, dissolve thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	Colony diameter 0.5-1 mm, circular with entire margin, opaque, grayish-white on the obverse, flat morphology, smooth surface, moist texture, easily picked, Gram-negative (red), rod-shaped.
Sharing mode	Public welfare sharing