BeNa Culture Collection
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| Culture medium | BNCC353940 |
| Description | 3.5% Sodium Chloride Agar Medium |
| Composition | Beef Extract:3.0g, Peptone:10.0g, NaCl:35.0g, Agar:15.0g, pH:7.2-7.4(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic; |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, dissolve thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | Colony diameter 0.5-1 mm, circular with entire margin, opaque, grayish-white on the obverse, flat morphology, smooth surface, moist texture, easily picked, Gram-negative (red), rod-shaped. |
| Sharing mode | Public welfare sharing |
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