Escherichia coli|316872 |BNCC

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Escherichia coli (Migula) Castellani and Chalmers

Subculture procedure	① Prepare 1-2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into a freeze-drying tube, dissolve and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions	37 ° C; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white front, flat shape, smooth surface, bright surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Description

1. Name : Escherichia coli

2. BNCC No.: 316872

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Nutrient agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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