Escherichia coli|316872 |BNCC

Essential Information Related Products

Escherichia coli (Migula) Castellani and Chalmers

Culture medium	BNCC360925
Description	Nutrient Agar with 100μg/mL Ampicillin
Composition	Beef Extract:3.0g, Peptone:10.0g, NaCl:5.0g, Agar:20.0g, pH:7.0(25℃)
Growth conditions	37 ° C; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into a freeze-drying tube, dissolve and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white front, flat shape, smooth surface, bright surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing