Bacillus subtilis subspecies|191554 |BNCC

Essential Information Related Products

Bacillus subtilis subsp. subtilis

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 plates as specified above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol lamp, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 2-4 mm,Shape: Circular,Margin: Irregular,Transparency: Opaque,Color: Grayish-white,Elevation: Flat,Surface: Rough,Texture: Dry
Sharing mode	Public welfare sharing