Salmonella enterica subsp. enterica Potsdam serotype|192093 |BNCC

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Salmonella enterica subsp. enterica serovar Potsdam

Subculture procedure	① Prepare 1-2 plates as specified above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol lamp, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	Size: Moderate，Color: Grayish-white，Shape: Circular，Margin: Regular，Moistness: Moist，Smoothness: Smooth，Transparency: Opaque，Elevation: Raised
Sharing mode	Public welfare sharing

Salmonella potsdam

No. 192093

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: Freeze-dried tube for 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice： if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, nutrient gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of NB liquid media or two NA agar plates.

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 20h NB bacterial fluid is turbid, bacterial fluid scribing plate colonies are obviously typical
colony morphology:	size: general color: off-white shape: round edge: neat wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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