Bacillus subtilis|195956 |BNCC

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Bacillus smithii

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	55 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grey White Rise: Flat Surface: Bright and Smooth Texture: Wet and easy to pick up, G+(blue purple), Bacillus, Purity: Pure
Sharing mode	Public welfare sharing

1.Description

1. Name：Bacillus smithii

2. BNCC No.：195956

3.Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. R2A agar medium: tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast powder 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3 agar 12 peptone 0.25 glucose 0.5g,pH7.2±0.2. 121℃,15min

2. Atmosphere：aerobic

3. Temperature：55 ℃,24h

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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