Streptomyces coelicolor|336594 |BNCC

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Streptomyces coelicolor (Müller) Waksman et Henrici

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	30 ℃; 3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 2-4mm, circular, with irregular edges, opaque, white on the front, raised in the middle, rough on the surface, hard in texture, dry in texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Streptomyces coelicolor

Storage conditions : 2~8 ℃

No. : 336594

Product Format : freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth Conditions :30 ℃, aerobic, Gao Shi No. 1 medium, 5-7 days. Gao Shi No.1 culture medium: 20g of soluble starch, 0.5g of sodium chloride, 0.01g of ferrous sulfate, 1.0g of potassium nitrate, 0.5g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 15g of agar, 1.0L of distilled water, pH 7.3±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation.When the germ grows, it can be used.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability. in 5-7 days strain layer is obvious, and colony is typical
colony morphology: (above)	size: 2-4mm shape: round edge: irregular transparency: opaque Color: milky white uplift: middle raised surface: bright texture: dry
conclusion	good viability, no abnormal colony morphology, qualified

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