Lactobacillus acidophilus|336636 |BNCC

Essential Information References Certificate Related Products

Lactobacillus acidophilus

Subculture procedure	①Prepare 1-2 of the above plates (placed in an anaerobic environment for 24 hours of deoxygenation in advance) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water or liquid culture medium (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L/piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 24-48 hours; anaerobic (best liquid culture effect)
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with irregular edges, white on the front, raised in the middle, smooth and bright on the surface, moist texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Lactobacillus acidophilus

storage conditions: 2~8 ℃

No. 336636

product form: freeze dried, 200ul

Validity: 6 years

biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions :37 ℃, anaerobic, MRS medium, 24-48h. MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water

Recovery steps:

(1)Prepare a φ18mm test tube of 10ml liquid medium and 2 plates(Put in an anaerobic conditions for deoxygenation for 24h in advance);

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viability:	good viability. in 24-48 hour, liquid medium is turbid, strain layer is obvious; colony is typical on marked plate
colony morphology: (as shown below)	size: 1-2mm; Shape: round; Edge: irregular; Transparency: opaque color: off-white; Protuberance: middle bulge; Surface: rough; & nbsp; Texture: dry
Conclusion:	good viability, no abnormal colony morphology, qualified

Download certificate