Beauveria bassiana|117565 |BNCC

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Beauveria bassiana (Bals.-Criv.) Vuill.

Subculture procedure	① Prepare 1 test tube containing 5-10 mL of liquid culture medium or sterile water and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The small filamentous fungus forms distinct colonies on comprehensive PDA medium, exhibiting white, dense, and low-lying mycelium. The reverse side of the medium shows a pale yellow color.
Sharing mode	Public welfare sharing

Beauveria bassiana

Storage conditions: 2~8 ℃

No. 117565

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28℃, aerobic, comprehensive PDA, 3-5 days, comprehensive PDA: potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0±0.2. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plate

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 3-5days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 3-5 days plate colony is obvious
colony morphology: (above)	small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white, dense and low, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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