BeNa Culture Collection
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| Culture medium | BNCC352233 |
| Description | Potato glucose agar |
| Composition | Potato Infusion Powder:6.0g, Glucose:20.0g, Agar:20.0g, pH:5.6±0.2(25℃) |
| Growth conditions | 28 ℃, 7-10 days, aerobic |
| Subculture procedure | ① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows. |
| Storage conditions | Liquid nitrogen |
| Safety level | 1 |
| morphology | The mycelium exhibits radial growth and is well-developed, with aerial hyphae appearing grayish-white. Upon initial germination, grayish-white mycelium rapidly spreads circularly across the medium. After forming a circular colony, the margin remains white, while the central region gradually darkens, eventually turning brown to black. The hyphae are septate and branched, with older hyphae displaying a darker coloration. |
| Sharing mode | Public welfare sharing |
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