Botrytis cinerea|119822 |BNCC

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Alternaria solani Sorauer

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃, 7-10 days, aerobic
Storage conditions	Liquid nitrogen
Safety level	1
morphology	The mycelium exhibits radial growth and is well-developed, with aerial hyphae appearing grayish-white. Upon initial germination, grayish-white mycelium rapidly spreads circularly across the medium. After forming a circular colony, the margin remains white, while the central region gradually darkens, eventually turning brown to black. The hyphae are septate and branched, with older hyphae displaying a darker coloration.
Sharing mode	Public welfare sharing

Alternaria solani Sorauer

Storage conditions: 2~8 ℃

No. 119822

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions : 28°C, aerobic, integrated PDA, 7-10 days. Comprehensive PDA: 1.0L potato cooking liquid, 20.0g glucose, 3.0g KH2PO4, 1.5g MgSO4 7H2O, trace amount of vitamin B1, 20.0g agar, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:
(1) Prepare 1-2 pieces of PDA plates;
(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;
(4) Lay flat the small pieces to the center of the agar plate;
(5) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 7 days, plate colonies are obvious
colony morphology:	small filamentous fungi, the colony is obvious on the integrated PDA medium, gray-white, dense and vigorous hyphae, spreading and growing to the edge of the plate, and the back of the medium is black.
Conclusion:	good viability, no abnormal colony morphology, qualified

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