Candida albicans|259769 |BNCC

Essential Information References Certificate Related Products

Candida albicans (Robin) Berkhout

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	30 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 2-4mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth on the surface, moist texture, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Candida albicans

Storage conditions : 2~8 ℃

No. : 259769

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level :1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28-30 ℃, aerobic, YM medium. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2,121 ℃,15min.

Recovery steps:
(1)Prepare 1-2 YM plates;
(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/ YM plate;
(4)Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 40h YM plate colony is obvious
Colony morphology: (pictured above)	Size: Medium Shape: Round Edge: Neat Edge Transparency: Opaque Color: milky white uplift: raised surface: smooth and bright texture: moist
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

Download certificate