Hemolytic Staphylococcus|139156 |BNCC

Essential Information References Certificate Related Products

Staphylococcus haemolyticus

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	30 ℃; 24-48 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front and milky white on the back, raised in the middle, smooth on the surface, moist texture, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Azospirillum brasilense

Storage conditions: 2~8 ℃

No.: 139156

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30°C, aerobic, nitrogen-fixing medium, 18-24h. Nitrogen fixation medium: KH2PO4 0.2g,K2HPO4 0.8g,MgSO4.7 H2O 0.2g,CaSO4.2 H2O 0.1g,FeCl3 trace, Na2MoO4.2H2O trace, yeast extract 0.5g, mannitol 20.0g, agar 15.0g, distilled water 1.0 L,pH 7.2 sterilization.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a agar plate evenly.

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 24-48h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	size: 2-4mm shape: round edge: irregular transparency: opaque color: white uplift: middle convex surface: bright and smooth texture: moist and viscous
Conclusion	good viability, no abnormal colony morphology, qualified

Download certificate