Fusarium graminearum|145491 |BNCC

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Subculture procedure	① Prepare 2 tablets; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. Dissolve it thoroughly and then transfer it back to the test tube. Mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions	28 ℃; 3-5 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	White on the front, spreading and growing, producing white spores, yellow on the back
Sharing mode	Public welfare sharing

Aspergillus brasiliensis

No.: 145491

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, comprehensive PDA agar medium: potato boiling solution 1.0L, glucose 20.0g,KH₂PO₄3.0g,MgSO₄。7H₂O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 5d liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	the colony is obvious on the comprehensive PDA medium, and the colony is white and powdery, dry and vigorous, spreading on the surface of the plate.
conclusion:	good viability, no abnormal colony morphology, qualified

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