Nitrogen fixing bacteria|335805 |BNCC

Essential Information Certificate Related Products

Azotobacter sp.

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	30 ℃; 24-48 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 2-4mm, Shape: Circular, Edge: Neat, Transparency: Transparent, Color: Colorless Transparent, Bulge: Middle Bulge, Surface: Bright and Smooth, Texture: Wet and Viscous
Sharing mode	Public welfare sharing

1.Description

1. Name: Azotobacter sp.

2. BNCC No.: 335805

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Medium composition:KH2PO4 0.2g，K2HPO4 0.8g，MgSO4.7H2O 0.2g，CaSO4·2H2O 0.1g，Na2MoO4.2H2O trace, yeast extract 0.5g, mannitol 20g,FECL3 Tract trace, distilled water 1000ml, agar 15g,PH7.2

2. Culture time: 24-48h

3, Temperature: 28-30 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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