Penicillium flavum|336234 |BNCC

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Penicillium chrysogenum Thom

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	It grows well on Czapek agar, with white, velvety mycelium at the margin and a bluish-green center that turns yellow in later stages. The reverse side of the colony is pale yellow.
Sharing mode	Public welfare sharing

Penicillium chrysogenum Thom

Storage conditions : 2~8 ℃

No. : 336234

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :25-28 ℃, aerobic, Cha's agar, 5-7 days. Cha's agar: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water, pH 6.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 2 plates;

(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability, in 5-7 days strain layer is obvious and colony is typical
colony morphology: (above)	filamentous fungi have obvious colonies on Cha's agar medium, hyphae are flourishing and growing, the edge mycelium is white, the texture is fluffy, the conidia structure is large, and blue-green spores are produced.
conclusion	good viability, no abnormal colony morphology, qualified

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