Streptomyces densiflora|153118 |BNCC

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Streptomyces pactum

Subculture procedure	① Prepare 1-2 plates of the aforementioned fresh medium.② Open the lyophilized vial inside a biosafety cabinet; flame the top of the vial with an alcohol lamp, then promptly apply sterile water to crack it open, followed by breaking the vial with forceps.③ Aspirate 0.5 mL of liquid medium into the lyophilized vial to fully dissolve the contents, then transfer the solution back into the liquid culture tube and mix thoroughly.④ Draw 0.2 mL of the bacterial suspension and inoculate it onto the Agar plate, spreading evenly. Repeat this step to prepare two plates.⑤ Incubate the plates under the aforementioned culture conditions until microbial growth is observed, after which the culture is ready for use.
Growth conditions	28 ℃, 24-48 hours, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 2-4mm, circular, with irregular edges, opaque, white on the front, wrinkled on the surface, hard in texture, producing gray white spores, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Streptomyces pactum

No. 153118

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, Streptomyces medium No. 2, 24-48h. Streptomyces medium No. 2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare the above 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 24-48h strain layer become obvious, colony is typical on the streaked plate.
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle raised surface: smooth texture: firm and hard
Conclusion:	good viability, no abnormal colony morphology, qualified

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