Aspergillus oryzae|336549 |BNCC

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Aspergillus oryzae(Ahlburg)Cohn

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 3-5 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on the comprehensive PDA medium, and the mycelium of the agar layer is white in the early stage, spreading and growing to cover the entire agar plate. In the later stage, they produce golden yellow spores, and the back of the medium is light yellow
Sharing mode	Public welfare sharing

Aspergillusoryzae(Ahlburg)Cohn

Description

1. BNCC No.: 336549

2. Biosafety level: 4

Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

comprehensive PDA:20% potato juice 1L, glucose 20g, KH2PO4 3g, mgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

2. Atmosphere: aerobic

3. Temperature: 25-28 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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