Penicillium purpureum|185641 |BNCC

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Penicillium steckii

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 3-5 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	It exhibits spreading growth and produces abundant spores, which are gray in color. The colony has an irregular margin and is opaque, with a gray obverse side. It is flat in elevation, with a rough and dull surface, and a dry texture. Purity: pure.
Sharing mode	Public welfare sharing

Penicillium chrysalis

Storage conditions : 2~8 ℃

No. : 185641

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, PDA,3-5 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g ( not included in liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) The plate was cultured under the above-mentioned culture conditions, and the bacteria were grown and used.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows

Item	test results
viability	good viability, in 4days strain layer become obvious
colony morphology:	small filamentous fungi have obvious colonies on PDA medium. The hyphae of the plate layer are white in the early stage, low, flat and fine, and then gradually become light green spores, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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