Fusobacterium necrophorum subspecies|336998 |BNCC

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Fusobacterium necrophorum subsp.necrophorum

Subculture procedure	① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, extract all the dissolved solution, and transfer it into 1-2 agar plates (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 48-72h; anaerobic
Storage conditions	-80 ℃
Safety level	2
morphology	Size: 2-4mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grey Rise Degree: Middle Rise Surface: Bright and Smooth Texture: Moist and easy to pick up
Sharing mode	Public welfare sharing

1.Description

1. Name: Fusobacterium necrophorum subsp.necrophorum

2. BNCC No.:336998

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Blond Agar (blood agar medium)

2. Atmosphere:anaerobic

3. Temperature: 37 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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