Corrupt Shewanella CN-32|337021 |BNCC

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Shewanella putrefaciens CN-32

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, light yellow on the front and yellow on the back, protruding in the middle, moist texture, easy to pick up, does not produce pigments, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing