Rhizopus gracilis|185840 |BNCC

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Rhizopus stolonifer

Subculture procedure	① Prepare 1 test tube containing 5-10 mL of liquid culture medium and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	A small filamentous fungus forms distinct colonies on comprehensive PDA medium. Initially, the mycelium appears grayish-white, dense, and vigorous, later producing black spores and exhibiting spreading growth toward the edges of the Agar plate. The reverse side of the medium shows a pale yellow color.
Sharing mode	Public welfare sharing

Rhizopus stolonifer

Storage conditions: 2~8 ℃

No.: 185840

Product format: freeze dried,200ul

Validity period: 24 months

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 2 pieces of PDA plates or 2 agar slants;

(2)Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; or transfer appropriate solution to
the agar slant.

(4)place the plates uprightly and agar slant obliquely under the above culture conditions for 5-7 days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability in 4 days plate colony is obvious
colony morphology:	The morphology of the bacteria is obviously visible in the integrated PDA agar medium, and the colony is dense cotton wool, white at the beginning, black spores at the end, and spread rapidly.
conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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