Thermophilic Bacillus subtilis|337107 |BNCC

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Geobacillus stearothermophilus

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	55 ℃; 24-48 hours; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, light yellow on the back, raised in the middle, smooth on the surface, moist texture, no pigment production, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name: Geobacillus stearothermophilus

2. BNCC No.:337107

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Nutritious gravy agar: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4·H2O when culturing Bacillus,which is beneficial to spore production. pH7.0. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 55 ℃

4.Notes:

1.Initiating the recovery, the freeze dried pellets shall be used up and shall not be retained. rehydrate the pellets with 0.2-0.25ml of liquid medium or sterile water. inoculate 1-2 agar plates with the solution. To avoid the failure of growth caused by insufficient solution, inoculation to 3 plates or more is not allowed.

2.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

3.Agar slant shall be inoculated asap, and long-term storage is not recommended.

4..Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

5.Users should ensure the safe storage and operation of the strain,and waste generated from the handling process should be discarded after high-pressure sterilization.

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